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![]() We will use the dockerized Hello example. Let's create a new folder called heroku-hello and inside a Dockerfile: mkdir heroku-hello It goes without saying that you will need R, shiny, and the Docker Engine installed too. ![]() Next time the CLI will log you in automatically. Then type heroku login which will prompt you to type in credentials. Use heroku -version to test if the CLI is ready to be used. To be able to communicate with Heroku from the command line, you'll use the Heroku Command Line Interface (CLI). You will need git to be installed locally to be able to make changes to the git repository we are going to create. You can still be on the free plan after verification, so it might make sense to do so. For example the number of apps (5 vs 100), custom domains, etc. Read more about the general Heroku pricing, the dyno types, and free dyno hours.Īccount verification and credit card info are not required for Heroku, but unverified accounts have limitations. To follow this tutorial, you'll need to sign up for Heroku. Heroku is really well suited for a Docker-based workflow because all Heroku applications run in a collection of lightweight Linux containers called dynos. As you have seen before, a Docker-based workflow with Shiny gives us an opportunity for local testing before deployment, which might also be very useful. What this means is that it is recommended to use a Docker-based stack to handle all the finicky system dependencies. ![]() Huge thanks to the maintainers for simplifying our lives and doing the heavy lifting for everyone else! The main caveat in their own words is that: If any of your R packages dependend on system libraries which aren't included by Heroku, such as libgmp, libgomp, libgdal, libgeos and libgsl, you should use the Heroku container stack together with heroku-docker-r instead. The main caveat for these R Buildpacks is of course not that these are community maintained. ![]() This R Buildpack GitHub repository is quite recent and supports packrat or renv based workflows for Shiny and Plumber. The Heroku landing page: R is not officially supportedīecause R is not one of the officially supported languages for Heroku, Buildpacks for R are all community maintained and might lag (e.g. The build mechanism is typically language-specific and is based on so-called Buildpacks. Heroku is also part of the Salesforce Platform, enabling enterprises to store and leverage customer data in Salesforce for full-cycle CRM engagement.Īn application (app) is the combination of its source code and the dependency description that determines how to build and run the application. Heroku is a cloud platform-as-a-service (PaaS) that lets you deploy, run and manage applications. In this post, you are going to learn how to deploy a dockerized Shiny application to Heroku using the Heroku Command Line Interface (CLI). Heroku is a popular option for hosting and scaling apps without managing infrastructure. ![]() ![]() ![]() To your proper, Dwarven Iron Helms break into shambling skeletons, whereas a line of Pikemen ready their spears for one last cost. To your left, towering giants rush into battle, bashing hordes of goblins high into the air with each swing of their bone clubs. You need a strong army at all times, and that means having your units leveled up and using only the best equipment – remember to upgrade your units and equipment, and if possible, craft new equipment to ensure your army is in the best shape possible.You stand solemn, overlooking the battlefield. Outposts in imminent danger, unfortunately, will be lost. One day is equivalent to one battle, and regardless whether you win or lose, you will be required to defend if your main castle is the one that’s under attack. If you see a ticker next to a land which is under attack, that represents the countdown to imminent danger. That way, you can earn the resources you need to upgrade what you’ve got, unlock new units to recruit, and earn a variety of other bonuses as you get closer to destroying the opposing castle. Yes, this is a castle defense game, just as the developer describes it, but in order to survive in Heroes and Castles 2, you also need to conquer new lands. ![]() These attacks vary depending on the weapon you’re using, but they do have a devastating effect in any case – they typically are capable of taking out multiple enemies, are at least able to stun them, and can penetrate shields should the enemy try to defend against them. You should always prioritize to unleash a Heavy Attack or two when fighting the enemy. If your Stamina is low, your attacks won’t be as effective, so you will want to conserve your Stamina by balancing aggressive and conservative attacks while on the battlefield. It’s cool to unleash powerful and swift attacks on the bad guy, but remember that these quick and/or powerful actions will drain your Stamina. That way, you can build a bigger army and do so faster – remember to spend those Command Points whenever possible, to ensure your army is large enough to survive against the enemy! 2. Summon Bannermen And Use Them To Your AdvantageĪs you progress through Heroes and Castles 2, you’ll find yourself against tougher enemies, which will require you to summon Bannermen, allowing you to earn Command Points at a faster rate Command Points are the points you’ll need to summon units. So with that having been said, let’s take you to a couple of useful Heroes and Castles 2 tips and cheats for building the ultimate castle and achieving victory against the enemy. There are several types of units you can recruit to your army, and various ways to build and upgrade your castle. You choose from one of three races, customize your hero to your liking, and defend your castle and conquer others with your powerful army of troops. Foursaken Media is offering a “mash-up” of third-person action RPGs, strategy, and castle defense gaming when describing the game. Unlike the original Heroes and Castles title, this sequel is only available on iOS at the moment. If you own a newer iPhone, meaning the iPhone 4s onwards, or a recent iPad model, then you’ll be able to step into the magical world of Heroes and Castles 2. ![]() ![]() ![]() We’ve gathered the settings of pretty much every high level player we could find, and we hope that you find it helpful. As there are a number of fan favorites across both the competitive scene, top teams, and high level streamers who may not be signed to a team, we compiled a list of both signed pros and top-tier players who may not be on a team. With all that being said, it comes as no surprise that we had to put together a list of the camera settings and controller setups that the top Rocket League pro players use. Boasting a consistent top 10-15 spot on Steam for concurrent players, and over 23 million hours played, Rocket League is a fan favorite for casual gamers and competitive players alike. Despite having access to powerful hardware, professional players still prefer keeping their settings at the. With a $1 million prize pool for the RLCS in 2018, Rocket League has some serious power in the scene, and doesn’t look to be going away anytime soon. Rocket League doesn’t require a state-of-the-art gaming PC to run smoothly. Rocket League has been a steady force in esports for years, of course with the premier event being the RLCS, or Rocket League Championship Series. That balance has allowed Rocket League to remain among the top games in the esports scene nearly since its release back in 2015. Rocket league offers a perfect balance of traditional sports, insane wacky mechanics, and hyper-balanced competitive play. Chatīind everything to “Savage!”, “Okay.”, “What a save!” and “Take the shot!”.What started off as a silly game of rocket boost powered cars playing soccer has turned into an esports mainstay over the years. ![]() ![]() This comes with a cost, as I can’t hear players jumping/boosting behind me. I play with sound off, as I can get distracted easily. Gameplay volume is the only sound that can contribute to your gameplay, so have everything else off (unless of course, you get enjoyment and immersion out of the other settings). ![]() Some people can be distracted by non-relevant sounds. I use this (I’m not colourblind), and I’ve seen some pros use it as well.
![]() ![]() He's been gaming since the Atari 2600 days and still struggles to comprehend the fact he can play console quality titles on his pocket computer. Combining it with a cantrip like green flame blade makes it a bit more viable. ![]() It does make for a fun melee option for non-Pact of the Blade warlocks. Oliver also covers mobile gaming for iMore, with Apple Arcade a particular focus. Master the art of combo attacks and strike unwitting foes in our Shadowblade Update, which is 1185:now live Become an Assassin today Trailers. Shadow blade could be used in your off-hand if you want to dual-wield, but none of your Blade Pact invocations work with it either. Current expertise includes iOS, macOS, streaming services, and pretty much anything that has a battery or plugs into a wall. It’s an increase in processing power and RAM, plus. Since then he's seen the growth of the smartphone world, backed by iPhone, and new product categories come and go. Tier 2, Shadow Ultra 24.99 per month: This bumps streaming up to support 4K resolutions, 144 fps at 1080p, and ray tracing compatibility. This awesome trailer features triple axles, disc brakes, 14 inch radial tires, and aluminum modular wheels. Fast-paced action platformer Shadow Blade joined Apple Arcade s catalog of over 235 games on Friday. Starting with the 3/16 inch steel wall tubing and 2 x 5 inch frame. ![]() Having grown up using PCs and spending far too much money on graphics card and flashy RAM, Oliver switched to the Mac with a G5 iMac and hasn't looked back. The SHAD 4 personal watercraft (PWC) trailer is built to haul four large jet ski's, and is loaded with smart features. At iMore, Oliver is involved in daily news coverage and, not being short of opinions, has been known to 'explain' those thoughts in more detail, too. He has also been published in print for Macworld, including cover stories. Shadow Blade - Universal - HD Gameplay Trailer 62,302 views 196 Dislike Share TouchGameplay 838K subscribers Shadow Blade by Crescent Moon Games Kuro is a young man on his. Oliver Haslam has written about Apple and the wider technology business for more than a decade with bylines on How-To Geek, PC Mag, iDownloadBlog, and many more. ![]() ![]() From Biscotti Otti Otti to Kush Mints, CAM’s selection, quality, and variety of flavors is a testament to their dedication to cannabis enthusiasts and the culture.But A CakeA pastry chef by trade with over 15 years of experience in food and health regulation and compliance. ![]() ![]() As a Sacramento-based cultivator, CAM has carved their own recognition and affinity among reputable brands like Connected California, Alien Labs, and Doja Pak. Xula has recently collaborated with fellow woman-owned brand, Barbari, to create the Period Daze Spliff to help with mother nature’s monthly gift.Shop highly rated dispensaries near youShowing you dispensaries near your citySee all .See all dispensariesSee all dispensariesCAM(CAM)Anna Willey is the owner of CAM (California Aristinal Medicine), a Sacramento based cannabis cultivator and delivery company. xula intersects their herbal index featuring ingredients like black cohosh root, motherwort, and kava root with a cannabinoid-based approach to women’s health. Karina Primelles and Mennlay Golokeh Aggrey are the dynamic duo leading xula, a Latin and Black-owned CBD brand. Gift of Doja factors in the terpene profiles, which are the essential oils produced by the plant, as well as the cannabinoid content which are the compounds that make a person feel lit into the curation process, ensuring that the consumer has a unique experience with every jar. (AdobeStock)xulaBased in Mexico City. curating cannabis flower and products based on their unique Appellations of Origin, soil care, and thoughtful cultivation process by the farmers. If you’re looking for an extra spice, you’ll have to try their Mama T’s Southern Hot Sauce.Gift of DojaGift of Doja is a women-of-color-led cannabis equity brand created by Bay Area cannabis entrepreneur Nina Parks, specializing in honoring the vibe of all beings and all things. Their signature Sweet Heat Texas Style BBQ Sauce is a perfect blend of tangy, smoky, and sweet flavors that can enhance your high & the taste of literally any foods you want. It’s hard to miss the cowgirl details all over Saucy’s brand. Despite the brand being based in California,Tess Taylor, founder of Saucy, is shamelessly Texan to the core and we love it. Keep an eye out for them to make even more noise in 2023.Dose of SaucyDose of Saucy is a Black woman-owned company that specializes in creating delicious THC-infused sauces & condiments. One thing they all have in common: Boss ladies leading the way.Jump to a sectionTop women owned cannabis brands in 2023Women with cannabis licenses to watch in 20232023’s top woman-led accessory, community, & service-based companiesTop women owned cannabis brands in 2023(AdobeStock)These 24 women-driven brands are on the rise. They’re broken up into three categories: Brands, license holders, and non-plant touching companies that specialize in accessories, services, and community-building. So while the cannabis plants that we all love to consume are all female, women are being left out of ownership roles in the industry the at large.To eliminate any excuse for “not knowing” any women-owned companies to support, we’ve compiled a list of 66 to explore throughout the rest of the year. (Miss Grass)Only 10% or less of businesses in Massachusetts and Ohio qualify as women-owned. And according to a report by MJBiz, the number of women in cannabis businesses is actually decreasing.Shoutout to Buy Weed From Women and Miss Grass for dropping their latest 4(THE)20 WOMEN’S MONTH BAG, which has shined a light on the shocking statistic that only one in five weed businesses is woman-owned. ![]() Last updated April 17, 2023(AdoeStock)Here are Leafly’s favorite women owned and operated cannabis companies to follow this year and beyond.Do you believe only 20% of cannabis companies are owned by women? It’s true.I confirm that this is not a shared device.By accessing this site, you accept the Terms of Use and Privacy Policy.Įxplore more topicsIndustry66 women-led cannabis companies to watch in 2023Nadir PearsonPublished on Ma Get cannabis.Where are you from?United StatesCanadaAre you 21 or older?not yetyou bet!Remember me for 30 days. See our Cookie Policy and Privacy Policy to learn moreBrowse LeaflyYour AccountJoin LeaflyAdvertise on LeaflyHomeDeliveryStrainsDispensariesCBD StoresProductsDealsNewsLearnCannabis 101Social impactDoctorsLab partners Download the Leafly outLearn. 66 women-led cannabis companies to watch in 2023 | Leafly LeaflyShop legal, local weed.Openadvertise on LeaflyLocating.changeDeliveryDispensariesDealsStrainsProductsCBDDoctorsCannabis 101Social impactWe use cookies for certain features and to improve your experience. ![]() ![]() ![]() And here is where the restrooms are located. Also yes you can bring water as well as outside food. Overwhelmed? Here are the top most recommended foods to eat at Petco Park. Local - Sushi at a baseball game? In San Diego, yes! Local - Freshest spot for “Cardiff Crack” tri-tip, hot bar+health options.Flatbreads, salads, fresh fruit, fresh juices, wine and craft beer ( Vegetarian friendly) Hot Hen spicy fried chicken sandos with fuego sauce, pickles and Fresno chile slaw on a brioche bun. Local - Acai bowls and margaritas, including new Padres Açaí Bowl and a Dragon Fruit Margarita. Local - Beef brisket, hot dogs and other bbq-sauced grillables Local - Grab the braised short rib taco, margaritas, birria, guacamole, al pastor burrito. Local - Pizza, Pizza Port Beer, Garlic Beer Buddies - no breath mints though! Local - Fresh grab-and-go sushi from the sister resaurant of Uni Sushi in Mission Valley. Hot dogs, sausages, nachos, a full bar, and beer New – Ousting the king of beers, Kona brews, Hawaiian bowls and other island-inspired grindz.ĭippin’ Dots ice cream, Lemonade and sweets like cotton candy, snow cones, churros Local - San Diego’s home grown fast-food chain is serving tacos, chicken strips, curly fries and other menu favorites plus a signature Purple Haze cocktail. Local - Locally sourced frozen popsicles with exotic flavors from lavendar lemonde to pineapple mojito pops.īeer and desserts, including freshly made caramel corn Local - Hamburgers from famous San Diego institution ( Vegetarian friendly) Local - Follow your nose to find borracho fries, Texas hot links, smoked brisket and pulled pork. Tarantino hot dogs, beer, soft drinks, nachos, pretzels. Mexican-style grilled corn cart with fuego sauce, cotija cheese, Tajin, and lime crema. Local - Cocktails from San Diego-based distilleryįood, Ballast Point’s full lineup including Hot Hen sandwiches, outdoor seating Naples-style pizzeria serving garlic knots, handmade meatball sandwich, pizza Local - Sweet, freshly baked gourmet cookies and ice cream sandwiches. Local - Some of the best carnitas in San Diego. Hot Dogs, Veggie Dogs, ballpark standards, pretzels, nachos, Coffee ( Vegetarian friendly) Local - Fresh fish at a ballpark? Only in San Diego! Local - The best sandwiches in North County San Diego come to Petco ( Vegetarian friendly) Sparkling, white and red wines by the glass or bottleĮlite seating and view with Brandt New York-style steak sandwich, BLTA, carne asada tacos, smoked portobello tacos and full bar Twenty four inches tall, 26 ounces of San Diego’s finest. ( Vegetarian friendly)īeer and a souvenir. Local - A fan favorite, try the Tijuana-style El Xolito bacon-wrapped hot dogs. Local-ish - Former San Diego County brewery pouring beer and serving Grand Ole BBQ sausage sandies. If your idea of food is simply “vegetables,” read our vegan and vegetarian guide.įiner ballpark food and drink, no view of the field If your idea of food is simply “beer,” read our Petco Park local and craft beer guide. For places to eat outside the stadium, view our guide to restaurants near Petco Park. What follows is a guide to the best peanuts, Cracker Jacks and fish tacos at San Diego’s famous ballpark to help you map out your in-game eating. Concession PricesCost may vary throughout the park and pricing subject to change, but here are the latest prices for 2023 to give you an idea of the current food and drink prices. Kona Big Wave Patio, joining last year’s new entries: Sambazon, Jack in the Box (yes it is local, founded in 1951 by a San Diegan), Holy Paleta frozen pops from Bonita.įans at Petco Park can access food and drink info through the MLB Ballpark App on their cellphone and use it to purchase meals, snacks, drinks and other items.įor increased safety, there will be no vendors walking the aisles selling beer, peanuts, popcorn, cotton candy, ice cream and other treats. New San Diego-based food & drink vendors in 2023 include Alpine Beer Taproom for local-ish beer on tap and local Grand Ole BBQ y Asado sausages New & LocalLocal flavor continues to be what Petco Park is all about. It’s always exciting to learn about what’s new. 2023 ConcessionsEach year, some venues bow out to make room for new food and beverage stands in the stadium. With only a few hours to work with, knowing what to eat is an essential part of your game plan. Home runs don’t always come easy but you can always count on Petco Park to deliver delicious concessions and tasty food options for Padres fans. ![]() ![]() ![]() Message me if you'd like to see some codes I've used or have any other questions. I would encourage you to seek further support in your research community and start fiddling around with RStudio and these various packages. I feel publishing with these types of R packages are more credible in the literature as well. I don't know where you are at in your career, but learning these tools has made me feel ahead of the game and more trusting of my results. Many others have stood where you're standing, resulting in a lot of blog posts etc. Although, command line analysis is quite difficult at times to learn and incredibly frustrating, it's not impossible. Therefore, I would advise to use CLC as more of a preliminary analysis tool. Once you have established a connection, you will need to install the necessary. When the installer completes, the CLC Workbench will start and prompt for license information (see below). Run the installer and follow the instructions on the screen. I ended up getting clearer results that I trusted and understood better after abandoning CLC and using DESeq and EdgeR (with DESeq being my preferred method). Download the CLC Workbench client for your operating system, Windows, MAC or Linux, 64 or 32 bit. Hi Cristina, I previously used CLC for RNA-Seq analysis and fiddled with lots of parameters, my final output delivered a low number of differentially expressed genes (DEG) that were uninformative. the oligo pair-annealing score." Not very helpful in my view.) the secondary structure score and parameters pertaining to oligo-pairs such as e.g. The algorithm considers both the parameters pertaining to single oligos, such as e.g. (Edit: From the help menu: "CLC Main Workbench employs a proprietary algorithm to rank primer and probe solutions. However, since you are quite free to set a multitude parameters, I guess that the hits are scored mainly based on fitting these as well as the alignment with the homolog sequences. I didn't really get any insight into the algorithm that CLC uses to determine primers in the manual. While checking the software options I saw that most people recommend Primer3 in this context. ![]() However, I didn't use it for TaqMan probes yet. This user manual can also be found in pdf format: UserManual. It offers convienient options to design primers from alignments that are able to discriminate between rather homolog sequences. Welcome to CLC Genomics Workbench 11.0.1 - a software package supporting your daily bioinformatics work. Since I am used to do primer design in the CLC Main Workbench that would obviously be my first choice. ![]() Applications CLC Genomics Workbench is used for genomics, transcriptomics, and epigenomics research. I want to design primers/probes for a TaqMan application. CLC Genomics Workbench supports all the major next-generation sequencing platforms, including Oxford Nanopore, PacBio, IonTorrent, BGI/MGI, Illumina, as well as Sanger. ![]() ![]() Turn off all your antivirus software before you act to prevent patch deletion.Download all the apps you need using Adobe Creative Cloud.You have to follow these easy simple steps It takes some really simple steps to download and install this app on your devices. It works like an original Adobe Software Application.Lifetime Activation and all software updates are acceptable.It also works with or without an Adobe account.This app is extremely reliable and works with or without an Adobe Account.This app works for Windows 64-bit operating system. ![]() You can use all the latest versions of Adobe CC both online and offline.Let us discuss some of the very key features here in this section It is ideal to patch all the latest versions of Adobe CC 20. This is one of the most reliable applications. Key Features of GenP – Universal Adobe Patcher After cracking all the adobe applications, the app will then be able to work both offline and online. ![]() GenP Adobe is an extremely outstanding patcher with a lot of features. Additionally, you would find this app user-friendly. It is very easy to use and is used to activate all the latest versions of Adobe CC 2019, 2010, and 2021. But rest assured that we regularly update our download links so be sure to check it from time to time.Īdobe CC GenP is an amazing universal patcher that cracks all the latest versions of Adobe Creative Cloud 2019, 2020, 2021 This is done in a very smooth and easy way. We can’t guarantee the frequency with which these patched exes will be released. If you want to update, you have to get an equally updated version of the exe file for your chosen CC 2019 software. If you do, the crack will become useless, and the program will go back to trial (if it isn’t expired already). ![]() With that said, you cannot update your copy of Adobe Photoshop CC 2019 or Adobe Illustrator CC 2019 (or any other CC 2019 program for that matter) once you have cracked it. ![]() ![]() Pathways are statistically tested for over-representation in the experimental gene list relative to what is expected by chance, using several common statistical tests that consider the number of genes detected in the experiment, their relative ranking and the number of genes annotated to a pathway of interest. A standard approach to addressing this problem is pathway enrichment analysis, which summarizes the large gene list as a smaller list of more easily interpretable pathways. Analyses often result in long lists of genes that require an impractically large amount of manual literature searching to interpret. However, analysis and interpretation of these data represent a major challenge for many researchers. The resulting data are growing exponentially, and their analysis helps researchers discover novel biological functions, genotype-phenotype relationships and disease mechanisms 1, 2. The complete protocol can be performed in ~4.5 h and is designed for use by biologists with no prior bioinformatics training.Ĭomprehensive quantification of DNA, RNA and proteins in biological samples is now routine. The protocol describes innovative visualization techniques, provides comprehensive background and troubleshooting guidelines, and uses freely available and frequently updated software, including g:Profiler, Gene Set Enrichment Analysis (GSEA), Cytoscape and EnrichmentMap. We describe how to use this protocol with published examples of differentially expressed genes and mutated cancer genes however, the principles can be applied to diverse types of omics data. ![]() The protocol comprises three major steps: definition of a gene list from omics data, determination of statistically enriched pathways, and visualization and interpretation of the results. We explain the procedures of pathway enrichment analysis and present a practical step-by-step guide to help interpret gene lists resulting from RNA-seq and genome-sequencing experiments. This method identifies biological pathways that are enriched in a gene list more than would be expected by chance. Pathway enrichment analysis helps researchers gain mechanistic insight into gene lists generated from genome-scale (omics) experiments. ![]() |